Potent inhibitory effect of silibinin from milk thistle on skin inflammation stimuli by 12-O-tetradecanoylphorbol-13-acetate

作者：Liu, Wenfeng^*; Li, Yonglian; Zheng, Xi; Zhang, Kun; Du, Zhiyun

来源：Food & Function, 2015, 6(12): 3712-3719.

DOI：10.1039/c5fo00899a

• NF-KAPPA-B
• SILYBUM-MARIANUM
• MOUSE SKIN
• CANCER
• CURCUMIN
• CYCLOOXYGENASE-2
• ANTIOXIDANT
• SUPPRESSION
• ACTIVATION
• EXPRESSION

Silibinin, a major polyphenol in milk thistle, has been reported to have multiple pharmacological activities; therefore, there is an urgent need to well understand how silibinin works on inflammation-associated skin diseases. We herein designed silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated skin inflammation to test its inhibitory effects. It was demonstrated that silibinin, applied topically onto mouse ears following TPA stimulation, effectively down-regulated the expressions of TPA-induced interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), necrosis factor-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Further mechanistic investigations indicated that silibinin suppressed the expression of I kappa B kinase (IKK) by inhibiting the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathway, and thereby suppressing TPA-stimulated nuclear factor-kappa B (NF-kappa B) activation. Promisingly, silibinin, used for transdermal application, may be a potent naturally occuring anti-inflammatory agent for the prevention of inflammation-associated skin diseases.

• 出版日期2015
• 单位广东工业大学; 广东轻工职业技术学院