科研之友
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摘要
Among the intracellular bacterial symbionts, the genus Wolbachia (Rickettsiaceae: Rickettsiales) is one of the most abundant taxa associated with reproductive systems of various insects. It is cytoplasmic inherited endosymbiont that induce various reproductive alterations. Characterization analysis has revealed that a number of arthropods harbour Wolbachia sp. estimates from 20-80%, with emphasize on Coleopterous insects (41 species have been reported so far). Therefore, in the context of broad existence and unique phenotypic actions to alter reproductive systems of various insect, we aimed to determine the intriguing possibility of this endosymbiont from one of the deadliest palm pest, i.e. red palm weevil (RPW), Rynchophorus ferrugineus (Curculionidae) from different geographical locations using polymerase chain reactions (PCR) with four sets of Wolbachia-specific primers (SPs) along with one pair of universal bacterial primer (BP). Parallel analysis was also carried out with Wolbachia strain isolated from whitebacked plant hopper, Sogatella furcifera (Homoptera: Delphacidae), as a positive control. Our analysis confirmed the absence of Wolbachia sp. across the various life stages of RPW reared in laboratory or captured from the field. Moreover, the phylogenetic analysis of all closely related Wolbachia-mediated weevils were compiled and retrieved from NCBI database indicates the extent of transfection of this bacterium into RPW for the future work on biological control of RPW. This study may facilitate to understand further evolutionary consequences of Wolbachia infection in weevils.
- 出版日期2018
- 单位福建农林大学
