Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction

作者：Liu Po Len; Chong Inn Wen; Lee Yi Chen; Tsai Jong Rung; Wang Hui Min; Hsieh Chong Chao; Kuo Hsuan Fu; Liu Wei Lun; Chen Yung Hsiang; Chen Hsiu Lin^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(43): 9480-9487.

DOI：10.1021/acs.jafc.5b01168

• resveratrol
• inflammation
• hypoxia
• reoxygenation
• surfactant

Reducing oxidative stress is crucial to prevent hypoxia-reoxygenation (H/R)-induced lung injury. Resveratrol has excellent antioxidant and anti-inflammatory effects, and this study investigated its role in H/R-induced type II pneumocyte dysfunction. H/R conditions increased expression of inflammatory cytokines including interleukin (IL)-1 beta (142.3 +/- 21.2%, P < 0.05) and IL-6 (301.9 +/- 35.1%, P < 0.01) in a type II alveolar epithelial cell line (A549), while the anti-inflammatory cytokine IL-10 (64.6 +/- 9.8%, P < 0.05) and surfactant proteins (SPs) decreased. However, resveratrol treatment effectively inhibited these effects. H/R significantly activated an inflammatory transcription factor, nuclear factor (NF)-kappa B, while resveratrol significantly inhibited H/R-induced NF-kappa B transcription activities. To the best of our knowledge, this is the first study showing resveratrol-mediated reversal of H/R-induced inflammatory responses and dysfunction of type II pneumocyte cells in vitro. The effects of resveratrol were partially mediated by promoting SP expression and inhibiting inflammation with NF-kappa B pathway involvement. Therefore, our study provides new insights into mechanisms underlying the action of resveratrol in type II pneumocyte dysfunction.

• 出版日期2015-11-4
• 单位中国医科大学