A thienoquinoxaline and a styryl-quinoxaline as new fluorescent probes for amyloid-beta fibrils

作者：Benzeid Hanane; Mothes Emmanuelle; Essassi El Mokhtar; Faller Peter^*; Pratviel Genevieve

来源：Comptes Rendus Chimie, 2012, 15(1): 79-85.

DOI：10.1016/j.crci.2011.10.009

• A beta-peptide
• Fibrils
• Fluorescent staining
• Lysozyme
• Quinoxaline

Simple and easy to prepare quinoxaline derivatives proved able to stain amyloid fibers such as aggregated lysozyme and A beta(1-40)-peptide by a fluorescence "turn on" mechanism. Thienoquinoxaline 1 allowed the detection of lysozyme and A beta(1-40) fibers at lambda = 555 and 532 nm, respectively, with excitation at lambda = 450 nm. Styryl-quinoxaline 2 stained lysozyme and A beta(1-40) fibers with fluorescence at lambda = 579 and 567 nm, respectively, upon excitation at lambda = 453 nm. The apparent K-d values for A beta(1-40) fibers were 77 and 294 nM for 1 and 2, respectively. The sensitivity of the aggregates detection assay with these new dyes was higher than that of thioflavin T. Considering their unique fluorescence properties compared to other dyes reported in the field, they can be considered as additional staining tools for the detection and studies of peptide/protein aggregation.

• 出版日期2012-1