Diagnostic Significance of Humoral Immune Responses to Recombinant Antigens of Mycobacterium tuberculosis in Patients with Pleural Tuberculosis

作者：Sumi S; Radhakrishnan V V^*

来源：Journal of Clinical Laboratory Analysis, 2010, 24(5): 283-288.

DOI：10.1002/jcla.20401

• Mycobacterium tuberculosis
• tuberculous pleural effusion
• malignant pleural effusion
• recombinant mycobacterial antigens
• ELISA

Mycobacterium tuberculosis bacilli are seldom demonstrated in tuberculous pleural effusion (TPE) by conventional bacteriological methods. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG against four mycobacterial recombinant antigens (ESAT-6, PlcA, HspX and Tb8.4) in 69 pleural fluids of patients with TPE and 71 patients with malignant pleural effusion. To increase the sensitivity of the assay, a multi-antigen cocktail containing all the above antigens were also used. IgG positivity in ELISA for PlcA, HspX, Tb8.4, ESAT-6 antigens and multi-antigen complex were 49.3, 60.8, 49.3, 53.6 and 75.4% respectively. Each one of the above four antigens and their multi-antigen cocktail were highly specific in distinguishing tuberculous and malignant pleural effusion. This new generation immunoassay will serve as a useful marker for the diagnosis of pleural tuberculosis patients in whom M. tuberculosis bacilli were not demonstrated by bacteriological methods. J. Clin. Lab. Anal. 24:283-288, 2010.

• 出版日期2010
• 单位河北医科大学