A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1

作者:Duckworth Owen W*; Markarian Dawn S; Parker Dorothy L; Harrington James M
来源:Journal of Microbiological Methods, 2017, 135: 11-13.
DOI:10.1016/j.mimet.2017.01.019

摘要

Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse phase absorption and size exclusion) approach.

  • 出版日期2017-4