X Chromosome of Female Cells Shows Dynamic Changes in Status during Human Somatic Cell Reprogramming

作者：Kim Kun Yong; Hysolli Eriona; Tanaka Yoshiaki; Wang Brandon; Jung Yong Wook; Pan Xinghua; Weissman Sherman Morton; Park In Hyun^*

来源：Stem Cell Reports, 2014, 2(6): 896-909.

DOI：10.1016/j.stemcr.2014.04.003

Induced pluripotent stem cells (iPSCs) acquire embryonic stem cell (ESC)-like epigenetic states, including the X chromosome. Previous studies reported that human iPSCs retain the inactive X chromosome of parental cells, or acquire two active X chromosomes through reprogramming. Most studies investigated the X chromosome states in established human iPSC clones after completion of reprogramming. Thus, it is still not fully understood when and how the X chromosome reactivation occurs during reprogramming. Here, we report a dynamic change in the X chromosome state throughout reprogramming, with an initial robust reactivation of the inactive X chromosome followed by an inactivation upon generation of nascent iPSC clones. iPSCs with two active X chromosomes or an eroded X chromosome arise in passaging iPSCs. These data provide important insights into the plasticity of the X chromosome of human female iPSCs and will be crucial for the future application of such cells in cell therapy and X-linked disease modeling.

• 出版日期2014-6-3