A spatial-resolved electrochemiluminescence aptasensor for carcinoembryonic antigen detection in a double-check mode

作者：Liu, Fu-Rao; Cao, Jun-Tao^*; Wang, Yu-Ling; Fu, Xiao-Long; Ren, Shu-Wei; Liu, Yan-Ming^*

来源：Sensors and Actuators B: Chemical , 2018, 276: 173-179.

DOI：10.1016/j.snb.2018.08.082

• Carcinoembryonic antigen
• Silver-polyamidoamine nanoparticles
• Resonance energy transfer
• CdS-C composites
• Luminol-gold nanoparticles

A spatial-resolved electrochemiluminescence (ECL) aptasensing platform for sensitive protein detection was constructed in a double-check mode. CdS-C composites as cathodic ECL emitters and luminol-gold nanoparticles (L-Au NPs) as anodic ECL emitters were immobilized on working electrode 1 (WE1) and working electrode 2 (WE2) of the dual-disk glassy carbon electrodes (DDCE), respectively. The platform was designed by integrating silver-polyamidoamine NCs-labeled biotin-aptamer (Ag-PAMAM-bio-Apt) with the ECL emitters on the DDCE. The in-situ consumption of co-reactants in the ECL system by Ag-PAMAM NCs resulted in the great ECL quenching on both WE1 and WE2. The resonance energy transfer between L-Au NPs and Ag-PAMAM NCs made the ECL on WE2 further decrease. In the presence of carcinoembryonic antigen (CEA), portions of Ag-PAMAM-bio-Apt detached from the DDCE because of the competitive reaction of CEA and cDNA toward to Ag-PAMAM-bio-Apt, generating an enhancement of ECL intensity. This strategy achieves the detection of CEA in a double-check mode with detection limits of 0.39 pg mL(-1) for the cathodic assay (WE1) and 0.20 pg mL(-1) for the anodic assay (WE2), respectively. Furthermore, the proposed method had been successfully applied to determine CEA in human serum sample with recoveries of 80.0-110.0%, showing great potential in cancer diagnosis.

• 出版日期2018-12-10
• 单位信阳师范学院