A Strategy to Design Hyperpolarized C-13 Magnetic Resonance Probes Using [1-C-13]-Amino Acid as a Scaffold Structure

作者：Nishihara Tatsuya; Kameyama Yutaka; Nonaka Hiroshi; Takakusagi Yoichi; Hyodo Fuminori; Ichikawa Kazuhiro; Sando Shinsuke

来源：Chemistry-An Asian Journal, 2017, 12(9): 949-953.

DOI：10.1002/asia.201700098

• amino acids
• biosensors
• dynamic nuclear polarization
• hyperpolarization
• NMR spectroscopy

Hyperpolarization is an emerging method that dramatically enhances NMR signal intensity. As a result of their increased sensitivity, hyperpolarized (HP) NMR molecular probes can be used to perform time-resolved spectroscopy and imaging in vitro and in vivo. It is, however, challenging to design such probes de novo. Herein, the [1-C-13]-amino acid is reported as a scaffold structure to design HP (CNMR)-C-13 molecular probes. The [1-C-13]-amino acid can be converted to various HP C-13 chemical probes that show sufficient chemical shift change by altering the chemical state of the nitrogen upon interaction with the target. Several previously reported HP probes could be explained by this design principle. To demonstrate the versatility of this approach, two -amino-acid-based HP C-13 chemical probes, sensitive to pH and Ca2+ ion, were developed and used to detect targets.

• 出版日期2017-5-4