Down-regulation of P-Glycoprotein Is Associated with Resistance to Cisplatin and VP-16 in Human Lung Cancer Cell Lines

作者：Wang, Jiarui; Wang, Huiling; Zhao, Long; Fan, Sufang; Yang, Zhonghai; Gao, Fei; Chen, Lixia; Xia, Gary Guishan; Molnar, Joseph; Wang, Qi^*

来源：Anticancer Research, 2010, 30(9): 3593-3598.

• Lung cancer
• drug resistance
• P-glycoprotein
• down-regulation

Aim: To investigate whether down-regulation of P-glycoprotein (P-gp) is correlated to resistance to cisplatin and VP-16 in four histopathological subtype cell lines of lung cancer (SK-MES-1, SPCA-1, NCI-H-460 and NCI-H-446). Materials and Methods: After pretreatment with or without verapamil, the P-gp expression was examined by means of RT-PCR and immunofluorescence. Cell survival on treatment with cisplatin and VP-16 was determined by MTT assay. Results: The expression of P-gp was clearly inhibited by verapamil in all four cell lines. Following pretreatment with verapamil, NCI-H-446 was more sensitive to cisplatin, while SPCA-1, NCI-H-460 and NCI-H-446 were more sensitive to VP-16 compared to the control. Conclusion: Down-regulation of P-gp is associated with intrinsic resistance to cisplatin in the NCI-H-446 and to VP-16 in SPCA-1, NCI-H-460 and NCI-H-446 cell lines. These findings indicate that down-regulation of P-gp may be helpful for the reversion of drug resistance in some lung cancer cell line subtypes.

• 出版日期2010-9
• 单位大连医科大学