Neuroprotective effects of carnosic acid on neuronal cells under ischemic and hypoxic stress

作者：Hou Chien Wei; Lin Yu Tzy; Chen Yi Ling; Wang Ya Hui; Chou Jui Ling; Ping Ling Yin; Jeng Kee Ching^*

来源：Nutritional Neuroscience, 2012, 15(6): 257-263.

DOI：10.1179/1476830512Y.0000000021

• Carnosic acid
• Hypoxia
• ROS
• NO
• Cytokine
• MAPKs
• Caspase-3
• COX-2

Ischemia/hypoxia induces oxidative stress which is associated with neurodegenerative diseases. The present study investigated protective mechanism of carnosic acid (CA) on ischemia/reperfusion and hypoxia-induced neuronal cell injury. The results showed that CA reduced 52% of the infarct volume from brains under ischemia/reperfusion in vivo and protected the PC12 cells from hypoxic injury in vitro. CA (1.0 mu M) enhanced cell viability, prevented lactic dehydrogenase (LDH) release, scavenged reactive oxygen species (ROS), increased superoxide dismutase activity, and attenuated Ca2+ release, lipid peroxidation, and prostaglandin E-2 production in hypoxic PC12 cells. In addition, CA also reduced nitric oxide (NO) and interleukine (IL)-1 and IL-6 production from activated BV-2 microglia. Furthermore, its effect on hypoxia-induced mitogen-activated protein kinases (MAPKs) signaling pathway and caspase-3 was examined. Extracellular signal-regulated protein kinases, c-jun NH2-terminal kinase, and p38 MAPK were activated during hypoxia. CA inhibited MAPKs, caspase-3, and COX-2 activation and correlated well with the diminished LDH release and apoptosis (TUNEL) in PC12 cells under hypoxia. Taken together, CA protected neuronal cells under ischemia/hypoxia through scavenging or reducing of ROS and NO, inhibiting COX-2 and MAPK pathways by anti-inflammatory and anti-oxidative properties.

• 出版日期2012-11