A simple method to generate oxidized phosphatidylcholines in amounts close to one milligram

作者：Zschoernig Kristin; Schiller Juergen^*

来源：Chemistry and Physics of Lipids, 2014, 184: 30-37.

DOI：10.1016/j.chemphyslip.2014.09.003

• Phosphatidylcholine
• Lipid (per)oxidation
• Thin-layer chromatography
• MALDI MS

Oxidized (phospho)lipids are of paramount interest from different reasons: beside their significant in vivo relevance, these products are often needed in the laboratory to study the response of selected cells to oxidized lipids. Unfortunately, the commercial availability of oxidized lipids is limited and scientists interested in studying the physiological impact of oxidized lipids are normally forced to prepare the required compounds by themselves. %26lt;br%26gt;We will show here that chain-shortened products of oxidized phosphatidylcholines (PCs) such as aldehydes and carboxylic acids can be easily (and in nearly quantitative yields) generated by the Fenton reaction (H2O2 + Fe2+) or the KMnO4-induced oxidation of the PC. Using the Fenton reaction and physiological saline, chlorinated oxidation products such as chlorohydrins are also readily available. Additionally, it will be shown that preparative thin-layer chromatography (TLC) is a convenient but simple method to isolate the individual oxidation products in reasonable yields and high purities: all relevant products could be successfully identified by matrix-assisted laser desorption and ionization (MALDI) mass spectrometry and the amounts of the oxidized products determined by a simple colorimetric assay.

• 出版日期2014-12
• 单位河北医科大学