A Biosensor Based on Swim Bladder Membrane as Substrate for Determination of Glucose

作者：Jia Wen Juan; Cui Miao; Zhang Yan; Shuang Shao Min^*

来源：Chinese Journal of Analytical Chemistry, 2011, 39(9): 1423-1426.

DOI：10.3724/SP.J.1096.2011.01423

• Glucose biosensor
• Swim bladder
• Glucose oxidase

A glucose biosensor based on the swim bladder immobilized glucose oxidase has been developed. The glucose concentration was quantified by the decrease of dissolved oxygen. The effects of enzyme loading, pH, buffer concentration and interference were studied. When the enzyme concentration was 2.0%, pH was 7.0, and the buffer concentration was 100 mmol/L, the biosensor showed a maximum response. The biosensor showed a wide linear response with a concentration range of 0.016 -1.2 mmol/L, fast response time (70 s), good repeatability (2.5%, n=10) and satisfactory reproducibility (1.2% n=3). The biosensor retained 88.5% of its initial response after 3 months, and the immobilized enzyme retained its 95% activity after 200 times used. The proposed biosensor method was successfully applied to the determination of glucose concentration in human blood serum, and the results were agreement with that obtained from a colorimetric assay kit method. The recoveries were between 95.2% and 106.3%.

• 出版日期2011-9
• 单位山西大学