APPLICATIONS OF NEXT-GENERATION SEQUENCING Sequencing technologies - the next generation

作者：Metzker Michael L^*

来源：Nature Reviews Genetics, 2010, 11(1): 31-46.

DOI：10.1038/nrg2626

Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.

• 出版日期2010-1