Determination of five anthraquinones in medicinal plants by capillary zone electrophoresis with beta-cyclodextrin addition

作者：Tian Kan; Zhang Huige; Chen Xingguo^*; Hu Zhide

来源：Journal of Chromatography A, 2006, 1123(1): 134-137.

DOI：10.1016/j.chroma.2006.04.021

• capillary zone electrophoresis
• beta-cyclodextrin
• anthraquinones
• medicinal plants
• apparent binding constants

A simple and rapid method for the simultaneous determination of five anthraquinone derivatives including aloe-emodin, emodin, chrysophanol, physcom and rhein in Rheum species and Polygonum cuspidatum was established by capillary zone electrophoresis (CZE) using beta-cyclodextrin (CD) as modifier and urea to enhance its solubility. The apparent binding constants of these derivatives with P-CD were evaluated. After an optimization study, the best conditions were selected using 35 mM phosphate buffer (pH 11.0) containing 20 MM P-CD and 2 M urea, applied voltage 20 kV and detection at 254 nm. Under such conditions, all of the five anthraquinones were baseline-separated within a short analysis time of 12 min with symmetrical peaks and high theoretical plate numbers (189 000-314 000). The RSD values of the migration times and peak areas were 0.6-1.1, 1.3-1.9% (intra-day) and 0.6-1.5, 1.3-2.8% (inter-day, for a 5-day period), respectively. The limits of detection for the analytes (S/N=3) were 0.33-0.62 mu g/ml. The recoveries were ranged from 93.37 to 107.69%. The proposed method was successfully applied to the determination of anthraquinones in ethanol extracts of two kinds of Rheum plants (R. palmatum and R. hotaoense) and P. cuspidatum.

• 出版日期2006-8-4
• 单位兰州大学