Ultrasensitive aptamer based detection of beta-conglutin food allergen

作者：Svobodova Marketa^*; Mairal Teresa; Nadal Pedro; Carmen Bermudo M; O'Sullivan Ciara K

来源：Food Chemistry, 2014, 165: 419-423.

DOI：10.1016/j.foodchem.2014.05.128

• beta-Conglutin binding aptamer (beta-CBA)
• beta-Conglutin allergen
• Lupine
• Apta-PCR

Lupine has been increasingly used in food applications due to its high nutritional value and excellent functional properties. However, there has been a response to the increasing number of severe cases of lupine allergies reported during the last decade, and as a result lupine was recently added to the list of substances requiring mandatory advisory labelling on foodstuffs sold in the European Union. In this paper we report the robust and ultrasensitive detection of the anaphylactic beta-conglutin allergen using Apta-PCR achieving a detection limit of 85 pM (25 ng mL(-1)). No cross-reactivity with other conglutins or plant species potentially used in lupine containing foodstuffs was observed. This robust method provides an effective analytical tool for the detection and quantification of the toxic beta-conglutin subunit present in lupine flour.

• 出版日期2014-12-15