An isoflavone compound daidzein elicits myoblast differentiation and myotube growth

作者：Lee Sang Jin; Tuan Anh Vuong; Go Ga Yeon; Song Yoo Jin; Lee Seulah; Lee Soo Yeon; Kim Shin Woong; Lee Jaecheol; Kim Yong Kee; Seo Dong Wan; Kim Ki Hyun; Kang Jong Sun; Bae Gyu Un^*

来源：Journal of Functional Foods, 2017, 38(04): 438-446.

DOI：10.1016/j.jff.2017.09.016

• Akt signaling
• Daidzein
• Muscle regeneration
• Muscular atrophy
• Myoblast differentiation
• MyoD

Preservation of muscle stem cell function is critical for muscle homeostasis and the regenerative capacity of stem cells declines with age, leading to muscle loss. Previously, daidzein, a natural isoflavone from Leguminosae, has been shown to prevent TNF-alpha-induced muscle atrophy. However, the molecular mechanisms of daidzein in these processes are currently unknown. In this study, we demonstrate a positive effect of black soybean extract (BS Ext) and daidzein on myoblast differentiation and myotube growth. Daidzein prevents dexamethasone-induced myotube atrophy through Akt activation. The promyogenic effect of BS Ext and daidzein is mediated through two promyogenic kinases, Akt and p38MAPK that in turn activates a key myogenic transcription factor MyoD. Additionally, BS Ext and daidzein promote myotube growth through AktimTOR/S6K pathway. Finally, daidzein augments MyoD-dependent myogenic conversion of mouse embryonic fibroblasts. Taken together, our data suggest that daidzein promotes myogenic differentiation and myotube hypertrophy through p38 and AktimTOR/S6K pathway.

• 出版日期2017-11