Vitamin D-3 suppresses morphological evolution of the cribriform cancerous phenotype

作者：Deevi Ravi K; McClements Jane; McCloskey Karen D; Fatehullah Aliya; Tkocz Dorota; Javadi Arman; Higginson Robyn; Durban Victoria Marsh; Jansen Marnix; Clarke Alan; Loughrey Maurice B; Campbell Frederick C^*

来源：Oncotarget, 2016, 7(31): 49042-49064.

DOI：10.18632/oncotarget.8863

• colorectal cancer
• molecular oncology
• vitamin D receptor gene
• morphology
• prognosis

Development of cribriform morphology (CM) heralds malignant change in human colon but lack of mechanistic understanding hampers preventive therapy. This study investigated CM pathobiology in three-dimensional (3D) Caco-2 culture models of colorectal glandular architecture, assessed translational relevance and tested effects of 1,25(OH)(2)D-3, the active form of vitamin D. CM evolution was driven by oncogenic perturbation of the apical polarity (AP) complex comprising PTEN, CDC42 and PRKCZ (phosphatase and tensin homolog, cell division cycle 42 and protein kinase C zeta). Suppression of AP genes initiated a spatiotemporal cascade of mitotic spindle misorientation, apical membrane misalignment and aberrant epithelial configuration. Collectively, these events promoted "Swiss cheese-like" cribriform morphology (CM) comprising multiple abnormal "back to back" lumens surrounded by atypical stratified epithelium, in 3D colorectal gland models. Intestinal cancer driven purely by PTEN-deficiency in transgenic mice developed CM and in human CRC, CM associated with PTEN and PRKCZ readouts. Treatment of PTEN-deficient 3D cultures with 1,25(OH)(2)D-3 upregulated PTEN, rapidly activated CDC42 and PRKCZ, corrected mitotic spindle alignment and suppressed CM development. Conversely, mutationally-activated KRAS blocked 1,25(OH)(2)D-3 rescue of glandular architecture. We conclude that 1,25(OH)(2)D-3 upregulates AP signalling to reverse CM in a KRAS wild type (wt), clinically predictive CRC model system. Vitamin D could be developed as therapy to suppress inception or progression of a subset of colorectal tumors.

• 出版日期2016-8-2