An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects

作者：Gurgel Rodrigues Jose Arievilo; Lino de Queiroz Ismael Nilo; Gomes Quindere Ana Luiza; Vairo Bruno Cunha; de Souza Mourao Paulo Antonio; Barros Benevides Norma Maria

来源：Ciencia Rural, 2011, 41(4): 634-639.

• green alga
• sulfated polymers
• biological events
• coagulation system
• thrombosis

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP 1, SP 2 and SP 3). We investigated the effects of SP 2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP 2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP 2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC (50) similar to 10.0 mu g mL(-1)) and this mechanism of interaction is different when compared to other studied Caulerpa polysaccharides. SP 2 exhibited antithrombotic effects at doses of 1.0 and 2.0mg kg(-1) body weight, but at higher doses (> 2.0mg kg(-1) body weight) this polysaccharide revert the antithrombotic property. No hemorrhagic effect (2.0mg kg(-1)) was observed. As occurs with red algae SPs, these results indicate that green algae SPs are also capable of exhibiting different in vivo properties.

• 出版日期2011