Disrupting androgen production of Leydig cells by resveratrol via direct inhibition of human and rat 3 beta-hydroxysteroid dehydrogenase

作者：Li Ling; Chen Xiaomin; Zhu Qiqi; Chen Dongxin; Guo Jingjing; Yao Wenwen; Dong Yaoyao; Wei Jia; Lian Qingquan; Ge Ren Shan; Yuan Bo^*

来源：Toxicology Letters, 2014, 226(1): 14-19.

DOI：10.1016/j.toxlet.2014.01.022

• Steroidogenesis
• Resveratrol
• Rat Leydig cells
• Steroidogenic enzymes
• 3 beta-Hydroxysteroid dehydrogenase

Resveratrol is a polyphenol produced by several plants. It has been demonstrated that it has anti-inflammatory, antitumor, and anti-diabetic effects in animal models. However, its side effects are generally unclear. In the present study, we reported that resveratrol inhibited luteinizing hormone-stimulated androgen production in rat immature Leydig cells. Further analysis demonstrated that it was a competitive inhibitor of rat and human 3 beta-hydroxysteroid dehydrogenase with IC50 values of 3.87 +/- 0.06 and 8.48 +/- 0.04 mu M, respectively. The inhibition on 3 beta-hydroxysteroid dehydrogenase was specific since it did not inhibit another hydroxysteroid dehydrogenase 17 beta-hydroxysteroid dehydrogenase 3 at the highest concentration (100 mu M) tested. In conclusion, resveratrol potentially interferes with androgen biosynthesis of rat Leydig cells.

• 出版日期2014-4-7
• 单位温州医科大学; 同济大学