科研之友
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摘要
The synthesis of a new fluorescent probe 2-(3,7-dioxo-2-phenyl-3,7-dihydrochromeno[7,6-b][1,4]oxazin-9-yl)acetic acid succinimidyl ester was described. The reagent reacts easily with primary amines and forms highly fluorescent derivatives that can be separated and detected with an HPLC-fluorometric detector. The new reagent was evaluated using pentylamine as a model compound. The separation was achieved on a C-18 column using a mixture of acetonitrile: ammonium acetate buffer as the mobile phase. Statistical evaluation of the methodology described reveals good linearity, precision and accuracy, and high sensitivity. Application of the methodology can be used for the separation of four aliphatic arnmes (pentylamine, hexylamine, octylamine, and decylamine).
- 出版日期2004
