Methamphetamine and diazepam suppress antigen-specific cytokine expression and antibody production in ovalbumin-sensitized BALB/c mice

作者：Wey Shiaw Pyng; Wu Hsin Ying; Chang Fang Chia; Jan Tong Rong^*

来源：Toxicology Letters, 2008, 181(3): 157-162.

DOI：10.1016/j.toxlet.2008.07.015

• Methamphetamine
• Diazepam
• Antibody production
• Splenocyte
• Cytokine

Methamphetamine is a widely abused psychostimulant. Abusing methamphetamine causes various adverse effects, such as immune dysfunction. The present study investigated the effect of diazepam, a central depressant, on methamphetamine-induced immuncisuppression. BALB/c mice were daily administered with diazepam and methamphetamine (5 mg/kg of each), either alone OF in combination, for 5 consecutive days followed by sensitization with ovalbumin (OVA). Two days later the same dosing and sensitization regimen was repeated once. The production of serum anti-OVA antibodies, and the cellularity and functional activities of splenocytes were measured 7 days post the 2nd OVA sensitization. The results demonstrated that methamphetamine and/or diazepam significantly attenuated the production of OVA-specific IgM, IgG(1) and IgG(2a). Concordantly, splenocytes of mice administered with diazepam and/or methamphetamine produced less IL-4 and IFN-gamma upon ex vivo re-stimulation with OVA, as compared to the vehicle-treated control. In contrast, the cellularity, and metabolic activity of splenocytes were not altered by the drug treatment. These results indicated that the central depressant diazepam did not affect methamphetamine-mediated immunosuppression. Rather, both drugs markedly suppressed antigen-specific antibody production and T-cell reactivity.

• 出版日期2008-10-1
• 单位长春大学