UBE2T silencing inhibited non-small cell lung cancer cell proliferation and invasion by suppressing the wnt/β-catenin signaling pathway

作者：Liu, Juncai^*; Liu, Xiaochun

来源：International Journal of Clinical and Experimental Pathology, 2017, 10(9): 9482-9488.

• UBE2T
• NSCLC
• progression
• Wnt/beta-catenin

Background: UBE2T, a human E2 ubiquitin-conjugating enzyme, is overexpressed in some human cancers, suggesting the biological significance of UBE2T in cancer progression. However, its role in non-small cell lung cancer (NSCLC) progression is still unclear. This study was aimed to investigate the critical roles of UBE2T in NSCLC progression. Methods: QRT-PCR and Western blot were performed to explore the expression levels of UBE2T in NSCLC tissues and cell lines. Cell proliferation was detected using MTT assay and EdU assay. Cell invasion and migration were evaluated using transwell assay. Results: Our data showed that UBE2T expression was upregulated in NSCLC tissues and cell lines. Functionally, UBE2T knockdown suppressed NSCLC cell proliferation, migration and invasion in vitro. Mechanistic investigations revealed that UBE2T downregulation suppressed the activity of Wnt/beta-catenin signaling pathway. Conclusion: These results suggested that UBE2T play critical roles in the progression of NSCLC and could be a potential therapeutic target for the treatment of NSCLC patients.

• 出版日期2017
• 单位河南大学