A Platform for Preparation of Monodispersed Fluorescent Conjugated Polymer Microspheres with Core-Shell Structures

作者：Wang, Shangli; Zhao, Wei; Song, Jing; Cheng, Si; Fan, Li-Juan^*

来源：Macromolecular Rapid Communications, 2013, 34(1): 102-108.

DOI：10.1002/marc.201200560

• conjugated polymer
• flow cytometry
• fluorescent microspheres
• laser confocal microscopy
• monodispersed

A strategy to prepare stable monodispersed fluorescent microspheres is developed by modifying the Wessling method to synthesize poly(p-phenylenevinylene) (PPV) on the surface of a highly crosslinked polymer core. The positively charged PPV polymer precursors (pre-PPV) are adsorbed onto the core with negative charges on the surface and then the insoluble fluorescent PPVs form after thermal elimination. Each individual sphere is found to possess a very smooth surface with an even distribution of fluorescence by microscopic techniques. Very small coefficient of variance (CV) values of emission intensity (<4.0%) and size (<2.3%) are realized for microspheres prepared in the same batch. The spheres are demonstrated to have good thermal stability and photostability.

• 出版日期2013-1-11
• 单位苏州大学