Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment

作者：Wang JianGe; Su XiaoHua; Ji LiLi; Zhang BingYu; Hu ZanMin; Huang RongFeng; Tian YingChuan

来源：Chinese Science Bulletin, 2007, 52(2): 224-230.

DOI：10.1007/s11434-007-0034-2

• Populus xeuramericana 'Guariento'
• biolistic bombardment
• multi-gene
• co-transformation
• transgene

A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.

• 出版日期2007-1
• 单位江西农业大学; 中国科学院遗传与发育生物学研究所; 中国科学院微生物研究所; 中国农业科学院; 中国林业科学研究院林业研究所