A Cocktail of Thermally Stable, Chemically Synthesized Capture Agents for the Efficient Detection of Anti-Gp41 Antibodies from Human Sera

作者：Pfeilsticker Jessica A; Umeda Aiko; Farrow Blake; Hsueh Connie L; Deyle Kaycie M; Kim Jocelyn T; Lai Bert T; Heath James R^*

来源：PLos One, 2013, 8(10): e76224.

DOI：10.1371/journal.pone.0076224

We report on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60 degrees C.

• 出版日期2013-10-7