A novel low-temperature resistant alkaline lipase from a soda lake fungus strain Fusarium solani N4-2 for detergent formulation

作者：Liu Ruizhi^*; Jiang Xiaolu; Mou Haijin; Guan Huashi; Hwang HueyMin; Li Xiaoxia

来源：Biochemical Engineering Journal, 2009, 46(3): 265-270.

DOI：10.1016/j.bej.2009.05.016

• Enzyme
• Microbial
• Chromatography
• Biocatalyst preparation
• Detergent
• Alkaline lipase

The effects of temperature, pH, and various components in a detergent on the activity and stability of a lipase produced by a soda lake fungus strain Fusarium solani N4-2 were studied in a preliminary evaluation for use in detergent formulation. The molecular mass of the lipase was 31.6 kDa by SDS-PAGE after purification using acetone fractionation and Q-sepharose ion exchange technique. In aqueous solutions, the lipase showed maximal activity at pH 9.0 in a glycine-NaOH buffer at 30 degrees C. At 0 degrees C and 10 degrees C, 52.3% and 82.6% of its maximum activities were detained, respectively. Among the metal ions tested, additions of Cu(2+), Ca(2+), Mg(2+), Al(3+) and Mn(2+) ions were found to enhance the enzyme activity while Zn(2+), Ba(2+) and Hg(2+) ions showed inhibitory effects. Compared with the commercial lipase Lipolase (R), the enzyme showed better stability towards selected surfactants, commercial detergents, oxidizing agents and proteases. The remarkable resistance capability of the lipase makes it a potential additive for better detergent formulation.

• 出版日期2009-11-1
• 单位中国海洋大学