Activation of oxidative stress response by hydroxyl substituted chalcones and cyclic chalcone analogues in mitochondria

作者：Guzy J; Vaskova Kubalkova J^*; Rozmer Z; Fodor K; Marekova M; Poskrobova M; Perjesi P

来源：FEBS Letters, 2010, 584(3): 567-570.

DOI：10.1016/j.febslet.2009.11.098

• Cyclic chalcone analogue
• Mitochondria
• Respiration
• Glutathione
• Oxidative stress
• Hydroxyl radical

L We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased radical generation and possible covalent interaction of the compounds with cellular thiols resulted in glutathione (GSH) depletion and modulation of the investigated mitochondrial activities. Disruption of interconnected mechanisms as electron transport chain and energetic metabolism, ROS production and insufficiency of antioxidant defensive system could lead to induction of cell death.

• 出版日期2010-2-5