Antioxidant properties of polysaccharide fractions with different molecular mass extracted with hot-water from rice bran

作者:Zha Xue Qiang; Wang Jun Hui; Yang Xue Fei; Liang Hao; Zhao Ling Ling; Bao Su Hua; Luo Jian Ping*; Xu Ying Yin; Zhou Bin Bin
来源:Carbohydrate Polymers, 2009, 78(3): 570-575.
DOI:10.1016/j.carbpol.2009.05.020

摘要

Polysaccharides were extracted with hot-water from rice bran and precipitated with 40% ethanol (PW1), 60% ethanol (PW2) and 80% ethanol (PW3) in turn. PW1 and PW3 were composed of Glu, Man, Gal, Rib and Ara with molar percent of 54.1%, 10.5%, 21.7%, 7.4% and 6.3% for PW1 and 50.7%, 10.1%. 32.7%. 2.4% and 4.1% for PW3. Man was not observed in PW2. The molecular weight distribution of PW1, PW2 and PW3 ranged from 1.2 x 10(5) to 6.3 x 10(6) Da, 3.5 x 10(4) to 7.4 x 10(4) Da and 5.3 x 10(3) to 2.3 x 10(4) Da, respectively. Antioxidant activity tests revealed that PW1 showed good capability of scavenging superoxide radical, hydroxyl free radical and anti-lipid peroxidation at 1.0 mg/mL. In the meanwhile PW3 exhibited the good potential for reducing power, chelating ferrous ions, scavenging 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonate), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide effects.