Binding event measurement using a chip calorimeter coupled to magnetic beads

作者：Ahmad L M; Towe B; Wolf A; Mertens F; Lerchner J^*

来源：Sensors and Actuators B: Chemical , 2010, 145(1): 239-245.

DOI：10.1016/j.snb.2009.12.012

• Chip-calorimetry
• Biosensor
• Biotin-streptavidin reaction
• DNA hybridization

Chip calorimetry is a promising method for the label-free detection of biomolecules. Miniaturized calorimetric devices using stagnant droplets require sample volumes in the nano-liter range, but exhibit some limitations regarding volume specific signal resolution, baseline stability, and evaporation. In this paper, we describe the application of a silicon chip based flow-through calorimeter for the detection of biotin and aptamers. To achieve high sensitivity, receptors coupled to magnetic beads were used. Streptavidin served as a receptor for the detection of biotin. For the first time, the chip-calorimetric response to DNA hybridization has been successfully demonstrated. For these experiments, the biotinylated complement of the DNA oligomer was coupled to the streptavidin surface of magnetic beads.

• 出版日期2010-3-4