科研之友
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摘要
We have modified a quick, inexpensive and less prone to contamination protocol by culturing the cryopreserved or fresh mycelium directly in 96-deepwell plate. The method facilitates concomitant assessment of ssDNA fungal diversity by asymmetric PCR (A-PCR) from a single extraction. The DNA yields from Fusarium spp. isolates was reasonably high, and a clear DNA band was frequently seen when 10 muL of the PCR product was run in agarose gel. The procedure can be completed in less than 4 h and 96 samples can be processed at the same time.
- 出版日期2003-6
