Anti-tumor effects of Rubratoxin B on cell toxicity, inhibition of cell proliferation, cytotoxic activity and matrix metalloproteinase-2,9

作者：Wang Tao^*; Zhang Yi; Wang Yi; Pei Yue hu

来源：Toxicology in Vitro, 2007, 21(4): 646-650.

DOI：10.1016/j.tiv.2007.01.001

• Penicillium purpurogenum
• Pyricularia oryzae
• Rubratoxin B
• cell cycle inhibitor
• cytotoxic activity
• anti tumor

Terrestrial fungi are a prolific source of metabolites with significant biological activities. Many important anticancer, antifungal and antibacterial chemotherapeutics are either microbial metabolites or semisynthetic derivatives. Investigating the metabolites of fungi may increase the chance of finding novel compounds. A bioassay-guided fractionation of soil fungus Penicillium purpurogenum fermentation, yielded the metabolite Rubratoxin B using morphological deformation of Pyricularia oryzae mycelia. Analysis by flow cytometry showed that Rubratoxin B inhibited the cell cycle progression of tsFT210 cells in the G2/M phase at the concentration of 24 mu M. Cytotoxic activity analysis showed that Rubratoxin B has a cytotoxic activity and IC50 was 67.3 +/- 1.4 mu M on human fibrosarcoma cell (HT 1080), and Rubratoxin B exhibited inhibitory activities against matrix metalloproteinase (MMP)-2 and 9 on HT 1080 cells with an inhibitory rate of 61.5% and 74.7% at 30 mu M, respectively.

• 出版日期2007-6
• 单位沈阳药科大学; 中国中医科学院