Discrimination of rapeseeds (Brassica napus L.) based on the content of erucic acid by H-1 NMR

作者：Han, Jiao; Lu, Changming; Li, Yinping; Deng, Zhiwei; Fu, Boqiang^*; Geng, Zhufeng^*

来源：European Food Research and Technology, 2016, 242(3): 441-447.

DOI：10.1007/s00217-015-2555-2

• Rapeseed
• Erucic acid
• Nuclear magnetic resonance
• Hierarchical cluster analysis
• T test

Rapeseeds (Brassica napus L.) are major source of edible oil-consumed in daily life. However, the high erucic acid content in some rapeseeds is harmful to the human health. So it is essential to explore a convenient and rapid method to identify which content of erucic acid is less than 5 %. Since the derivation procedure of traditional identification method (gas chromatography) is cumbersome, it consumes much time in analysis. In this work, a rapid method based on nuclear magnetic resonance (NMR) technology is developed. Firstly, H-1 NMR spectra of rapeseeds extract were collected, and then H-1 NMR data were analyzed by hierarchical cluster analysis and t test. The results indicated that statistically significant differences (p < 0.05) were found between high-erucic-acid and low-erucic-acid groups. Therefore, the new method can effectively distinguish LEAR from high-erucic-acid rapeseed with convenient sample pretreatment and data acquisition, which offer great potential for LEAR screening.

• 出版日期2016-3
• 单位中国计量科学研究院; 北京师范大学; 中国农业科学院油料作物研究所