The effects of gold nanoparticles functionalized with beta-amyloid specific peptides on an in vitro model of blood-brain barrier

作者：Ruff J; Huewel S; Kogan Marcelo J; Simon Ulrich^*; Galla Hans Joachim^*

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2017, 13(5): 1645-1652.

DOI：10.1016/j.nano.2017.02.013

• Hollow gold nanospheres
• Gold nanorods
• Blood-brain barrier
• Impedance spectroscopy

We studied the effect of gold nanoparticle (AuNP) size, surface charge, concentration and morphology on the integrity of the blood-brain barrier (BBB) in a well-established in vitro model set-up. We focused on the effect of peptide functionalized hollow gold nanospheres and gold nanorods, which selectively bind to amyloidogenic beta-amyloid structures. These AuNP conjugates have already been successfully tested as photothermal absorbers for potential application in Alzheimer's disease (AD) therapy in an in vitro set-up, but may exhibit a low passage through the BBB due to their overall negative charge. Our results show that: (i) small (1.4 nm) AuNPs strongly affects the BBB integrity, (ii) negative surface charge impedes BBB passage, and (iii) this charge effect caused by the peptide is compensated by covalent coupling to a polyethylene glycol ligand stabilizing the particles in diluted manner.

• 出版日期2017-7