Urine Levels of Defensin alpha 1 Reflect Kidney Injury in Leptospirosis Patients

作者：Chagan Yasutan Haorile; Chen Yue; Lacuesta Talitha Lea; Leano Prisca Susan A; Iwasaki Hiroko; Hanan Firmanto; Taurustiati Delsi; Ohmoto Yasukazu; Ashino Yugo; Saitoh Hiroki; Kiyomoto Hideyasu; Suzuki Yasuhiko; Telan Freda O Elizabeth; Hattori Toshio

来源：International Journal of Molecular Sciences, 2016, 17(10): 1637.

DOI：10.3390/ijms17101637

• leptospirosis
• defensin alpha 1
• neutrophil gelatinase-associated lipocalin (NGAL)
• kidney injury
• N-acetyl-beta-D-glucosidase (NAG)

Leptospirosis is a zoonotic disease whose severe forms are often accompanied by kidney dysfunction. In the present study, urinary markers were studied for potential prediction of disease severity. Urine samples from 135 patients with or without leptospirosis at San Lazaro Hospital, the Philippines, were analyzed. Urine levels of defensin alpha 1 (uDA1) were compared with those of neutrophil gelatinase-associated lipocalin (uNGAL) and N-acetyl-beta-D-glucosidase (uNAG). Serum creatinine (Cr) was used as a marker of kidney injury. The levels of uDA1/Cr, uNGAL/Cr, and uNAG/Cr were positive in 46%, 90%, and 80% of leptospirosis patients, and 69%, 70%, and 70% of non-leptospirosis patients, respectively. In leptospirosis patients, the correlation of uDA1/Cr, uNGAL/Cr and uNAG/Cr levels with serum Cr were r = 0.3 (p < 0.01), r = 0.29 (p < 0.01), and r = 0.02 (p = 0.81), respectively. uDA1/Cr levels were correlated with uNGAL/Cr levels (r = 0.49, p < 0.01) and uNAG/Cr levels (r = 0.47, p < 0.0001) in leptospirosis patients. These findings suggest that uDA1, uNGAL, and uNAG were elevated in leptospirosis patients and reflected various types of kidney damage. uDA1 and uNGAL can be used to track kidney injury in leptospirosis patients because of their correlation with the serum Cr level.

• 出版日期2016-10