A RAPID AND SENSITIVE in situ RNA HYBRIDISATION METHOD FOR OIL PALM TISSUES

作者：Siew Eng Ooi^*; Fong Chin Lee; Ong Abdullah Meilina

来源：Journal of Oil Palm Research, 2012, 24: 1235-1239.

• DIG
• in situ hybridisation
• plants
• SDS

We report here an in situ RNA (ribonucleic acid) hybridisation protocol with modified post-hybridisation procedures tested on tissues from two plants, oil palm and Arabidopsis thaliana. This protocol involves shorter post-hybridisation washes with SDS (sodium dodecyl sulphate) buffers, replacing the standard SSC (sodium chloride-sodium acetate-sodium citrate) and formamide buffers. This modified protocol was tested with a few genes, and contrary to the results from previously used protocols, clearer distinguishable signals were detected with antisense probes compared with the sense probes on hybridised tissue sections. The protocol also reduces the time taken by the previous standard protocol by approximately 6 hr and uses fewer reagents, thus saving time as well as costs.

• 出版日期2012-4