Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by pi-pi Stacking Interactions

作者：Le Devedec Frantz^*; Houdaihed Loujin; Allen Christine^*

来源：Jove-Journal of Visualized Experiments, 2016, (116): e54422.

DOI：10.3791/54422

• Chemistry
• Issue 116
• anionic polymerization
• block copolymer micelles
• phenyl glycidyl ether
• doxorubicin
• drug delivery
• pi-pi stacking interactions

In this study, an amphiphilic copolymer that includes a core-forming block with phenyl groups was synthesized by living anionic polymerization of phenyl glycidyl ether (PheGE) on methoxy-polyethylene glycol (mPEG-b-PPheGE). Characterization of the copolymer revealed a narrow molecular distribution (PDI < 1.03) and confirmed the degree of polymerization of mPEG(122)-b-(PheGE)(15). The critical micelle concentration of the copolymer was evaluated using an established fluorescence method with the aggregation behavior evaluated by dynamic light scattering and transmission electronic microscopy. The potential of the copolymer for use in drug delivery applications was evaluated in a preliminary manner including in vitro biocompatibility, loading and release of the hydrophobic anti-cancer drug doxorubicin (DOX). A stable micelle formulation of DOX was prepared with drug loading levels up to 14% (wt%), drug loading efficiencies > 60% (w/w) and sustained release of drug over 4 days under physiologically relevant conditions (acidic and neutral pH, presence of albumin). The high drug loading level and sustained release is attributed to stabilizing pi-pi interactions between DOX and the core-forming block of the micelles.

• 出版日期2016-10