Detection of Altered Extracellular Matrix in Surface Layers of Unstable Carotid Plaque: An Optical Spectroscopy, Birefringence and Microarray Genetic Analysis

作者：Korol Renee M^*; Canham Peter B; Liu Li; Viswanathan Kasinath; Ferguson Gary G; Hammond Rob R; Finlay Helen M; Baker Henry V; Lopez Cecilia; Lucas Alexandra R

来源：Photochemistry and Photobiology, 2011, 87(5): 1164-1172.

DOI：10.1111/j.1751-1097.2011.00960.x

Erosion and rupture of surface layers in atherosclerotic plaque can cause heart attack and stroke; however, changes in luminal surface composition are incompletely defined. Laser-induced fluorescence spectroscopy (LIFS), with limited tissue penetration, was used to investigate the surface of unstable carotid plaque and correlated with microscopy, birefringence and gene expression. Arterial matrix collagens I, III and elastin were assessed in unstable plaques (n = 25) and reference left internal mammary arteries (LIMA, n = 10). LIFS in addition to selective histological staining with picrosirius red, Movat pentachrome and immunostaining revealed decreased elastin and increased collagen I and III (P < 0.05) in carotid plaque when compared with LIMA. Within plaque, collagen I was elevated in the internal carotid region versus the common carotid region. Polarized light microscopy detected layers of aligned collagen and associated mechanical rigidity of the fibrous cap. Microarray analysis of three carotid and three LIMA specimens confirmed up-regulation of collagen I, III and IV, lysyl oxidase and MMP-12. In conclusion, LIFS analysis coupled with microscopy revealed marked regional differences in collagen I, III and elastin in surface layers of carotid plaque; indicative of plaque instability. Birefringence measurements demonstrated mechanical rigidity and weakening of the fibrous cap with complementary changes in ECM gene expression.

• 出版日期2011-10