A single molecule approach to mRNA transport by a class V myosin

作者：Sladewski Thomas E; Trybus Kathleen M^*

来源：RNA Biology, 2014, 11(8): 986-991.

DOI：10.4161/rna.29947

• Myo4p
• She3p
• She2p
• ASH1 mRNA
• mRNA transport
• mRNA localization
• messenger ribonucleoprotein
• mRNP
• messenger ribonucleoprotein

mRNA localization ensures correct spatial and temporal control of protein synthesis in the cell. We show that an in vitro single molecule approach, using purified recombinant full-length proteins and synthesized mRNA, provides insight into the mechanism by which localizing mRNAs are carried to their destination. A messenger ribonucleoprotein (mRNP) complex was reconstituted from a budding yeast class V myosin motor complex (Myo4p-She3p), an mRNA-binding adaptor protein (She2p), and a localizing mRNA (ASH1). The motion of the mRNP was tracked with high spatial (approximate to 10nm) and temporal (70ms) resolution. Using this bottom-up methodology, we show that mRNA triggers the assembly of a high affinity double-headed motor-mRNA complex that moves continuously for long distances on actin filaments at physiologic ionic strength. Without mRNA, the myosin is monomeric and unable to move continuously on actin. This finding reveals an elegant strategy to ensure that only cargo-bound motors are activated for transport. Increasing the number of localization elements (zip codes) in the mRNA enhanced both the frequency of motile events and their run length, features which likely enhance cellular localization. Future in vitro reconstitution of mRNPs with kinesin and dynein motors should similarly yield mechanistic insight into mRNA transport by microtubule-based motors.

• 出版日期2014-8