An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

作者：Mackay Ian J^*; Bansept Basler Pauline; Barber Toby; Bentley Alison R; Cockram James; Gosman Nick; Greenland Andy J; Horsnell Richard; Howells Rhian; O'Sullivan Donal M; Rose Gemma A; Howell Phil J

来源：Genes Genomes Genetics, 2014, 4(9): 1603-1610.

DOI：10.1534/g3.114.012963

• quantitative genetics
• genome-wide association scan
• single nucleotide polymorphism
• QTL mapping
• recombinant inbred line (RIL)
• high-density genotyping
• Multiparent Advanced Generation Inter-Cross (MAGIC)
• multiparental populations
• MPP

MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F-7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.

• 出版日期2014-9-1