Membrane association of monotopic phosphoglycosyl transferase underpins function

作者：Ray Leah C; Das Debasis; Entova Sonya; Lukose Vinita; Lynch Andrew J; Imperiali Barbara^*; Allen Karen N^*

来源：Nature Chemical Biology, 2018, 14(6): 538-+.

DOI：10.1038/s41589-018-0054-z

Polyprenol phosphate phosphoglycosyl transferases (PGTs) catalyze the first membrane-committed step in assembly of essential glycoconjugates. Currently there is no structure-function information to describe how monotopic PGTs coordinate the reaction between membrane-embedded and soluble substrates. We describe the structure and mode of membrane association of PglC, a PGT from Campylobacter concisus. The structure reveals a unique architecture, provides mechanistic insight and identifies ligand-binding determinants for PglC and the monotopic PGT superfamily.

• 出版日期2018-6
• 单位MIT