NADPH oxidase activation is required for migration by LIGHT in human monocytes

作者：Heo Sook Kyoung; Yun Hyun Jeong; Park Won Hwan; Park Sun Dong^*

来源：Biochemical and Biophysical Research Communications, 2008, 371(4): 834-840.

DOI：10.1016/j.bbrc.2008.04.184

• LIGHT-HVEM interaction
• NADPH oxidase activation
• migration
• innate immunity
• inflammatory response

Homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus (HSV) glycoprotein D (gD) for herpes virus entry mediator (HVEM; TR2) (LIGHT), a ligand of herpes virus entry mediator (HVEM), increased reactive oxygen species (ROS) and enhanced the destruction of bacteria in human monocytes. In this study, rhLIGHT was found to increase the expression of the chemokine receptors, chemokine receptor 1 (CCR1) and CCR2, as well as to accelerate the migration activity of human monocytes. Additionally, rhLIGHT was found to increase ROS via NADPH oxidase p47(phox) phosphorylation, which was found to be required for LIGHT-induced NF-kappa B activation, CCR1 and CCR2 expression, migration and IL-8 and TNF-alpha production. Taken together, these results indicate that NADPH oxidase activation is required for rhLIGHT-induced migration in human monocytes.

• 出版日期2008-7-11