Modification of Proteins with Cyclodextrins Prevents Aggregation and Surface Adsorption and Increases Thermal Stability

作者：Prashar Deepali; Cui DaWei; Bandyopadhyay Debjyoti; Luk Yan Yeung^*

来源：Langmuir, 2011, 27(21): 13091-13096.

DOI：10.1021/la203271u

This work describes a general approach for preventing protein aggregation and surface adsorption by modifying proteins with beta-cyclodextrins (beta CD) via an efficient water-driven ligation. As compared to native unmodified proteins, the cyclodextrin-modified proteins (lysozyme and RNase A) exhibit significant reduction in aggregation, surface adsorption and increase in thermal stability. These results reveal a new chemistry for preventing protein aggregation and surface adsorption that is likely of different mechanisms than that by modifying proteins with poly(ethylene glycol).

• 出版日期2011-11-1