Activation of the non-canonical NF-kappa B/p52 pathway in vascular endothelial cells by RANKL elicits pro-calcific signalling in co-cultured smooth muscle cells

作者：Harper Emma; Rochfort Keith D; Forde Hannah; Davenport Colin; Smith Diarmuid; Cummins Philip M^*

来源：Cellular Signalling, 2018, 47: 142-150.

DOI：10.1016/j.cellsig.2018.04.004

• Endothelial cell
• Smooth muscle cell
• Calcification
• RANKL
• TRAIL
• NF-kappa B/p52

Background: The intimal endothelium is known to condition the underlying medial smooth muscle cell (SMC) layer of the vessel wall, and is highly responsive to receptor-activator of nuclear factor-kappa B ligand (RANKL) and tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), pro-calcific and anti-calcific agents, respectively. In this paper, we tested the hypothesis that RANKL-induced activation of endothelial NF-kappa B signalling is essential for pro-calcific activation of the underlying SMCs.
Methods: For these studies, human aortic endothelial and smooth muscle cell mono-cultures (HAECs, HASMCs) were treated with RANKL (0-25 ng/ml +/- 5 ng/ml TRAIL) for 72 h. Non-contact transwell HAEC:HASMC cocultures were also employed in which the luminal HAECs were treated with RANKL ( +/- 5 ng/ml TRAIL), followed by analysis of pro-calcific markers in the underlying subluminal HASMCs.
Results: Treatment of either HAECs or HASMCs with RANKL activated the non-canonical NF-kappa B/p52 and canonical NF-kappa B/p65 pathways in both cell types. In RANKL +/- TRAIL-treated HAECs, recombinant TRAIL, previously demonstrated by our group to strongly attenuate the pro-calcific signalling effects of RANKL, was shown to specifically block the RANKL-mediated activation of non-canonical NF-kappa B/p52, clearly pointing to the mechanistic relevance of this specific pathway to RANKL function within endothelial cells. In a final series of HAEC:HASMC transwell co-culture experiments, RANKL treatment of HAECs that had been genetically silenced (via siRNA) for the NF-kappa B2 gene (the molecular forerunner to NF-kappa B/p52 generation) exhibited strongly attenuated pro-calcific activation of underlying HASMCs relative to scrambled siRNA controls.
Summary: These in vitro observations provide valuable mechanistic insights into how RANKL may potentially act upon endothelial cells through activation of the alternative NF-kappa B pathway to alter endothelial paracrine signalling and elicit pro-calcific responses within underlying vascular smooth muscle cells.

• 出版日期2018-7