科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objective: The objective of our study is to identify the early molecular processes involved in osseointegration associated with a micro roughened and nanosurface superimposed featured implants. Materials and methods: Thirty-two titanium implants with surface topographies exhibiting a micro roughened (AT-II) and nanosurface superimposed featured implants (AT-I) were placed in the tibiae of 8 rats and subsequently harvested at 2 and 4 days after placement. Total RNA was isolated from cells adherent to retrieved implants. A whole genome microarray using the Affymetrix Rat Gene 1.1 ST Array followed by validation of select genes through qRT-PCR was used to describe the gene expression profiles that were differentially regulated by the implant surfaces. Results: While significant differences at the gene level were not noted when comparing the two-implant surfaces at each time point, the microarray identified several genes that were differentially regulated at day 4 vs. day 2 for both implant surfaces. A total of 649 genes were differentially regulated at day 4 vs. day 2 in AT-I and 392 genes in AT-II implants. Functionally relevant categories related to ossification, skeletal system development, osteoblast differentiation, bone development, bone mineralization and biomineral tissue development were upregulated and more prominent at AT-I (day 4 vs. day 2) compared to AT-II. Analysis of the downregulated.gene lists (day 4 vs. day 2) with average fold change >2 (were not statistically significant) revealed the biological processes involved with the inflammatory/immune response gene expression. The number of genes that were associated with the inflammatory/immune response category was greater for AT-I than AT-II. Conclusions: The presence of nanosurface features modulated in vivo bone response. Gene regulation implicating osteogenesis as well as the inflammatory/immune responses that occur as a function of surface topography may affect bone mass shortly after implant placement.
- 出版日期2013-1
