A plasmidic class 1 integron from five Pseudomonas aeruginosa clinical strains harbored aacA4 and nonsense-mutated cmIA1 gene cassettes

作者：Yan He; Shi Lei; Yamasaki Shinji; Li Xinhui; Cao Yicheng; Li Lin; Yanga Liansheng; Miyoshi Shin ichi^*

来源：Journal of Health Science, 2007, 53(6): 750-755.

DOI：10.1248/jhs.53.750

• integron
• drug resistance
• Pseudomonas aeruginosa

Five strains of multidrug-resistant Pseudomonas aeruginosa (P aeruginosa) were isolated from inpatients at a local hospital in China. The most frequent resistance was to cefoperazone, ciprofloxacin, ceftriaxone, cefotaxime, gentamicin, piperacillin, trimethoprim sulfamethoxazole, and tobramycin. These strains were found to contain the class 1 integron, in which the 2360-bp gene cassettes were flanked by 5'- and 3'-conserved segments. Sequence analysis revealed that the gene cassettes contained aacA4 and cmlA1 genes; however, the latter gene had a nonsense mutation resulting in the production of a truncated protein. To the best of our knowledge, this is the first report of a nonsense mutation in the cmlA1 gene. Moreover, the R aeruginosa strains showed identical profiles in pulsed-field gel electrophoresis, suggesting that they were derived from the same clone. These results emphasize the importance of controlling the spread of multidrug-resistant pathogens in hospitals.

• 出版日期2007-12
• 单位华南理工大学