Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12

作者：Hosaka Takeshi; Ohnishi Kameyama Mayumi; Muramatsu Hideyuki; Murakami Kana; Tsurumi Yasuhisa; Kodani Shinya; Yoshida Mitsuru; Fujie Akihiko; Ochi Kozo^*

来源：Nature Biotechnology, 2009, 27(5): 462-464.

DOI：10.1038/nbt.1538

We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.

• 出版日期2009-5