Diversity and phylogeny of Neofusicoccum species occurring in forest and urban environments in Portugal

作者：Lopes A; Barradas C; Phillips A J L; Alves A

来源：Mycosphere, 2016, 7(7): 906-920.

DOI：10.5943/mycosphere/si/1b/10

• Botryosphaeriaceae
• endophytic
• host-association
• ornamentals
• pathogenic

A collection of Neofusicoccum isolates was obtained from a large number of plant species, showing dieback and canker symptoms, in forest and urban environments in Portugal. A total of 351 isolates was characterised by BOX-PCR fingerprinting to evaluate their overall genetic diversity. Representatives of each group identified in this analysis were selected for multilocus sequence analyses, using sequences of the ribosomal internal transcribed spacer region (ITS rDNA) and partial sequences of the translation elongation factor 1-alpha (tef1) and beta-tubulin (tub2). Phylogenetic analysis of multilocus sequence data identified five species within the collection of isolates, namely N. australe, N. eucalyptorum, N. kwambonambiense, N. luteum, and N. parvum. Of these N. australe and N. eucalyptorum were the most frequent representing the vast majority of the isolates. Several new fungus-host associations were established for all of the Neofusicoccum species found. Here we report for the first time the occurrence of N. eucalyptorum on a host outside the family Myrtaceae. The results of this study show that the genus Neofusicocccum appears to be common and widespread on a broad range of hosts representing a potential threat to susceptible plants. Additionally, ornamental plants in urban environments are shown to be hosts of a diverse assemblage of Neofusicoccum species.

• 出版日期2016