An artificial extracellular matrix created by hepatocyte growth factor fused to IgG-Fc

作者：Azuma Koji; Nagaoka Masato; Cho Chong Su; Akaike Toshihiro^*

来源：Biomaterials, 2010, 31(5): 802-809.

DOI：10.1016/j.biomaterials.2009.09.105

• Hepatocyte growth factor
• ECM (extracellular matrix)
• Cell morphology
• HepG2
• c-Met
• Akt

The design of artificial extracellular matrices (ECM) has attracted much attention in tissue engineering and regenerative medicine as well as in molecular biology research. A recombinant hepatocyte growth factor (HGF), fused to an immunoglobulin G (IgG) Fc region (abbreviated as AeHGF-Fc) was constructed and confirmed by Western blot assay. Almost similar amounts of HepG2 cells adhered to AeHGF-Fc-coated surface compared to collagen-coated one with large morphological changes. Immobilized AeHGF-Fc continuously activated Akt in HepG2 cells whereas Akt activation induced by soluble HGF rapidly decreased with time, indicating that immobilized AeHGF-Fc follows different signal transduction pathways compared to soluble HGF.

• 出版日期2010-2