A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle

作者：Xu, Yao; Shi, Tao; Zhou, Yang; Liu, Mei; Klaus, Sebastian; Lan, Xianyong; Lei, Chuzhao; Chen, Hong^*

来源：Scientific Reports, 2018, 8(1): 1724.

DOI：10.1038/s41598-018-20177-8

• SATELLITE CELLS
• FUNCTIONAL-CHARACTERIZATION
• STEM-CELLS
• TRAITS
• GROWTH
• ZNF219
• SNPS
• IDENTIFICATION
• ASSOCIATION
• MUTATIONS

Paired box 7 (PAX7) gene regulates the conversion of muscle satellite cells into myogenic cells and participates in multi-step processes in myogenesis. Expression levels of PAX7 are decisive for its regulatory function. Previous reports revealed that PAX7 were responsible for the developmental traits of muscle. The relationship of the PAX7 promoter variants and livestock phenotypic traits has not been fully elucidated. We detected a novel 10-bp insertion/deletion (indel) polymorphism in the bovine PAX7 promoter and revealed that the indel altered the binding of the transcriptional factor ZNF219. Luciferase reporter assay showed that deletion-deletion (Del-Del) genotype of the PAX7 gene showed 2.79-fold higher promoter activity than the insertion-insertion (Ins-Ins) genotype (P < 0.05), and ZNF219 overexpression significantly diminished the luciferase activity in Ins-Ins groups. Moreover, the expression of PAX7 and its down-stream genes were detected in fetal skeletal muscle of cattle with different PAX7 genotypes, where the Del-Del genotype also displayed high expression levels. Statistical association analysis demonstrated that this indel had significant effects on early growth traits in cattle. These findings provide a complete overview of the function of the PAX7 10-bp variant, which may have potential as a genetic marker for marker-assisted selection in improving economically significant traits of cattle.

• 出版日期2018-1-29
• 单位西北农林科技大学; 中国科学院成都生物研究所; 武汉科技大学